Wound Swab PowerPoint

Please download to get full document.

View again

of 40
68 views
All materials on our website are shared by users. If you have any questions about copyright issues, please report us to resolve them. We are always happy to assist you.

Download

Document Related
Document Description
1. Wound Swab Ashely Hamilton Angela Thetford MLT-2010 Professor Tiffany Gill CSM June 15, 2015 2. Collection 3. Wound Swab 4. Wound Swab Swabs tipped with Dacron or…
Document Share
Document Transcript
  • 1. Wound Swab Ashely Hamilton Angela Thetford MLT-2010 Professor Tiffany Gill CSM June 15, 2015
  • 2. Collection
  • 3. Wound Swab
  • 4. Wound Swab Swabs tipped with Dacron or Rayon polyester are optimum for recovery of infective bacteria.
  • 5. Primary Set Up
  • 6. Primary Set Up Nutritional Media for most bacterial growth Enriched media for fastidious bacteria Selective Media for Gram Negative bacteria 2 Bacterial Smears for Gram Stain
  • 7. Primary Set Up • BAP- Staphylococcus aureus and Streptococcus pyogenes will both grow on this plate. • CHOC- Media to enhance growth of fastidious organisms such as; Haemophilus influenzae, Neisseria gonorrhoeae and Neisseria meningitidis • MAC- Media selective for Gram Negative bacteria and differential for lactose fermenting and non lactose fermenting bacteria such as; E. coli and Enterobacter aerogenes. (Enteric bacteria)
  • 8. Media and Slide Inoculation
  • 9. Media/slide inoculation CDC,Atlanta
  • 10. Gram Stain
  • 11. Gram Stain • Perform a Gram Stain on your mixed colony smear. o Gram Stain reaction • Positive or Negative o Bacterial morphology • Cocci or Bacillus o Bacterial arrangement • Pairs, clusters, chains • Preliminary ID of bacteria
  • 12. Morphology and Arrangement
  • 13. Morphology and Arrangement Gram Positive Cocci , single, pairs and clusters Gram Positive Cocci, chains Gram Negative Bacilli, single and pairs Gram Negative Bacilli, pairs and chains
  • 14. Pathogens
  • 15. Pathogens • The 2 pathogens seen in this wound swab: • Gram Positive Streptococcus pyogenes • Gram Positive Staphylococcus aureus and/or MRSA (Methicillin Resistant Staphylococcus aureus)
  • 16. Media to detect pathogens • Blood agar (BAP) is a nutrient/differential growth medium and contains 5% sheep blood. Certain bacteria produce exotoxins called hemolysins, which act on the red blood cells to lyse, or break them down. This hemolysis pattern can help identify or narrow down a species of bacteria. •
  • 17. Morphology S. aureus and S. pyogenes
  • 18. Distinguishing Features Morphology Staphylococcus aureus Streptococcus pyogenes Gram positive Cocci Grape like clusters Gram positive Cocci Chains
  • 19. Colonial Growth S. aureus and S. pyogenes
  • 20. Distinguishing Features Colonial Growth Staphylococcus aureus Streptococcus pyogenes Golden yellow entire, smooth, convex colonies on BAP Double zone Beta hemolysis on BAP Opaque, pearly grey, smooth, flat colonies on BAP Wide zone of Beta hemolysis on BAP Facultative anaerobes will grow in CO2 incubator Growth period 18 to 24 hours @ 35 to 37C
  • 21. Normal Flora or Pathogen
  • 22. Normal Flora or Pathogen? Staphylococcus aureus and Normal Flora Streptococcus pyogenes and Normal Flora Contaminated Streptococcus pneumoniae plate with P disc
  • 23. Virulence Factors S. aureus
  • 24. Virulence Factors Staphylococcus aureus • Protein A-major component of cell walls, prevents opsonization from occurring • Extotoxins o Exfoliatin-cause scaled skin syndrome o Panton-Valentine-destroys leukocytes o TSST-1-Toxic Shock Syndrome • Exoenzymes o Hyaluronidase-destroys tissues o Hemolysins-destroy RBC o Coagulase-clot formation o Staphylokinase-dissolves clots
  • 25. Virulence Factors S. pyogenes
  • 26. Virulence Factors Streptococcus pyogenes • Fibrils on the surface that are composed of lipoteichoic acid and M protein: enables S. pyogenes to adhere to surfaces and functions as an antiphagocytic • Exotoxins o Streptolysin S and O: Beta hemolytic component o Streptococcal pyrogenic exotoxin: toxin that causes Toxic shock-like syndrome o Hyaluronidase: spreading factor o Streptokinase: digests fibrin
  • 27. Differential/Selective Media
  • 28. Differential/Selective Media • MSA-Mannitol salt agar o Selective-bacteria that can tolerate high salt concentrations o Differential-differentiates Staphylococcus aureus from Staphylococcus epidermidis
  • 29. Biochemical Testing S. aureus and S. pyogenes
  • 30. Biochemical Testing Staphylococcus aureus Streptococcus pyogenes • Catalase positive • Coagulase positive • Bacitracin Resistant • Catalase negative • PYR positive • CAMP negative • Bacitracin Sensitive
  • 31. Susceptibility Testing S. aureus and MRSA
  • 32. Susceptibility Testing Results Staphylococcus aureus • Sensitive o Ampicillin/Sulbactam o Amoxicillin/ Clavulante o Oxacillin o Naficillin o Cefazolin o Clindamycin • Resistant o Bacitracin o Penicillin MRSA • Sensitive o Vancomycin o Moxifloxacin o Doxycycline o TMX-SMX • Resistant o Penicillin o Ampicillin o Cefoxitin o Erythromycin o Clindamycin
  • 33. Susceptibility Testing S. pyogenes
  • 34. Susceptibility Testing Results Streptococcus pyogenes • Sensitive o Penicillin o Bacitracin • Resistant o Erythromycin o Tetracycline
  • 35. Indigenous Locations S. aureus and S. pyogenes
  • 36. Indigenous locations on the Human Body Staphylococcus aureus Streptococcus pyogenes • Lower GI • Skin • Nose • Pharynx • Mouth • Vagina • Pharynx • Mouth
  • 37. References
  • 38. References Alila Medical Media | Cell, Molecular Biology & Genetics Images. (n.d.). Retrieved June 15, 2015. www.alilamedicalimages.com Bacitracin test for Streptococcus pyogenes. (n.d.). Retrieved June 15, 2015. http://www.bacteriainphotos.com/bacteria-photo- gallery.html#streptococcus Blood agar (BAP) Bacterial Growth Medium. (n .d.) Retrieved http://www.scienceprofonline.org/microbiology/blood-agar- bacterial-growth-medium-BAP.html Cavanaugh, P., Lipsky, B., Bradbury, A., & Boteck, G. (2005, November 12).Treatment of diabetic foot ulcers. Retrieved October 4, 2015, from http://www.thelancet.com/journals/lancet/article/PIIS0140-6736(05)67699-4/ppt Culture Media - Blood Agar Plates and Hemolysis: Streptococcus and Other Catalase Negative Gram-Positive Cocci. (n.d.). Retrieved June 15, 2015. http://www.bacteriainphotos.com/bacteria-photo-gallery.html#streptococcus .
  • 39. References Engelkirk, P., & Engelkirk, J. (2008). Chapter 9: Gram Positive Cocci. In Laboratory diagnosis of infectious diseases: Essentials of diagnostic microbiology (pp. 213-237). Baltimore: Wolters Kluwer Health/Lippincott Williams & Wilkins. Engelkirk, P., & Engelkirk, J. (2008). Chapter 8: Introduction to medial Bacteriology. In Laboratory diagnosis of infectious diseases: Essentials of diagnostic microbiology(pp. 185-209). Baltimore: Wolters Kluwer Health/Lippincott Williams & Wilkins Engelkirk, P., & Engelkirk, J. (2008). Chapter 8: Introduction to medial Bacteriology. In Laboratory diagnosis of infectious diseases: Essentials of diagnostic microbiology (pp. 185-209). Baltimore: Wolters Kluwer Health/Lippincott Williams & Wilkins Estridge, B., & Reynolds, A. (2012). 7-3 Culture Techniques for Bacteriology. In Basic clinical laboratory techniques (6th Edition ed., pp. 703-710). Delmar Estridge, B., & Reynolds, A. (2012). 7-3 Culture Techniques for Bacteriology. In Basic clinical laboratory techniques (5th ed., pp. 711-722). Delmar
  • 40. References Estridge, B., & Reynolds, A. (2012). 7-4 The Gram Stain. In Basic clinical laboratory techniques (5th ed., pp. 727-732). Delmar CENGAGE. Staphylococcus aureus (n.d.) Retrieved June 15, 2015; http://www.bacteriainphotos.com/bacteria-photo-gallery.html#saureus Susceptibility testing. (n.d.). Retrieved June 15, 2015. http://cdn.iopscience.com/images/1367- 2630/14/7/073037/Full/nj424207f1_online.jpg
  • Search Related
    Next Slide

    Cover.doc

    We Need Your Support
    Thank you for visiting our website and your interest in our free products and services. We are nonprofit website to share and download documents. To the running of this website, we need your help to support us.

    Thanks to everyone for your continued support.

    No, Thanks