Nephelometry

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1. NEPHELOMETRY ASHLEY HAMILTON AND SHELIA EDOS MLT 1012 PROFESSOR DOMENICI FALL 2014 COLLEGE OF SOUTHERN MARYLAND 2. PURPOSE- WHAT NEPHELOMETRY IT USED FOR? ã…
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  • 1. NEPHELOMETRY ASHLEY HAMILTON AND SHELIA EDOS MLT 1012 PROFESSOR DOMENICI FALL 2014 COLLEGE OF SOUTHERN MARYLAND
  • 2. PURPOSE- WHAT NEPHELOMETRY IT USED FOR? • Nephelometry is a tool used in immunology laboratories for testing levels of proteins in your blood that are important for effective immune responses.
  • 3. PRINCIPLE- HOW IT WORKS • This test can quickly and accurately measure the level of immunoglobin proteins in blood, which are the proteins that help your body fight infection. • It is based on the principle that a dilute solution of small particles will scatter light passed through it, rather than simply absorbing it.
  • 4. PRINCIPLE- HOW IT WORKS • The tool used to measure the amount of scattered light is called a nephelometer. • If a substance is completely dissolved in a solution then there will be nearly no scattered light, leading to a low nephelometric value. • If the substance has not dissolved completely in a solution then light will be scattered, leading to a high nephelometric value.
  • 5. PRINCIPLE- HOW IT WORKS In example A you will see that all the solute has been dissolved into the solution, therefore there will be no scattered light leading to a low nephelometric value. In example B, the solute has not dissolved into the solution, therefore there is an increase in scattered light, leading to a high nephelometric value.
  • 6. APPLICATIONS • This technique is widely used in the laboratory and is easily automated. • Nephelometry is used for investigating different immunological problems such as liver disease, rheumatoid arthritis, leukemia, lymphoma, preeclampsia, inflammatory bowel disease, and chronic infections.
  • 7. APPLICATIONS- EXAMPLE • Patients who have an existing diagnosis of lupus will be monitored using nephelometry to measure certain complement protein levels because when the disease is in an active stage, these proteins are actively consumed. • Accurate measurement using nephelometry can assist the planning of therapy.
  • 8. REFERENCES FAQ: How Does Nephelometry Work? (2012, January 2). Retrieved September 10, 2014, from BMG Labtech website: http:// microplatereaders.blogspot.com/2012/01/faq-how-does- nephelometrywork.html Nephelometry. (2008). Retrieved September 10, 2014, from Virtual Laboratory website: http://www.science4u.info/virtuallab/autoimmunitynephelometrybackgroun d.htm Quantitative Nephelometry. (2012, June 5). Retrieved September 16, 2014, from The New York Times Health Guide website: http://www.nytimes.com/health/guides/test/quantitative-immunoglobulins- nephelometry/overview.html
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